Annunciation Catholic Academy campus is located on Jamestown Boulevard, west of Montgomery Road, in Altamonte Springs, Florida. Four two-story buildings comprising more than 65,000 sq. ft. are configured around a central courtyard featuring a statue of Our Lady. A separate, fully air conditioned gymnasium is located adjacent to the school.

The school has 22 classrooms; each features a special work area for students to pursue independent projects, work in small groups, or be tutored by a volunteer or teaching assistant. The school features dedicated classrooms for art, music and Spanish instruction. The science center has both an instructional classroom and a dedicated science laboratory.

The school’s media center has a fully automated management system and catalog, a centralized media retrieval system, a video and radio production room, and a central reading room. The school also contains a “cafetorium” – a multi-purpose room used as both a cafeteria and an auditorium.